The analysis of mammalian cell bioreactor cultivation involving real-time analytical techniques (in-line Kaiser RXN2 Raman spectroscopy and Hamilton Incyte capacitance-based cell density sensor) that can be used to determine the cell density and the concentration of nutrients and metabolites.
The Sykam Automatic Amino Acid Analyzer combines the advantages of the classical ion-exchange separation method with the modern technique of HPLC thus enabling the determination of the free amino acid concentration in complex biological samples (e.g cell-free harvest medium).
Octet K2 device based on label-free Bio-Layer Interferometry (BLI) provides robust monitoring of biomolecular interactions in real-time for the determination of kinetics, affinity, and antibody/protein quantitation.
The analysis of the therapeutic protein formulations is carried out by various techniques in our laboratory. Size exclusion chromatography, SDS-PAGE, turbidity, and kinetic characterization (Octet) can be used to determine the formation of protein aggregation or degradation.